Worthington offers 0.22µm filtered preparations of each type of Collagenase in pre-packaged form for direct reconstitution and use in cell isolation and culture procedures. Correlations between enzyme type and effectiveness with different tissues have been good, but not perfect, due in part to the variable parameters of use. Nevertheless most researchers consider the tissue-typing of crude collagenase lots to be a valuable service. A detailed description of the Worthington collagenase and contaminant assays can be found in the Worthington Enzyme Manual. In addition, tissue specific references and detailed isolation conditions can be found in the Worthington Tissue Dissociation Guide.
Uses: Crude collagenases are widely used in enzymatic primary cell isolation and tissue dissociation procedures. Most researchers employ either crude collagenase preparations such as Types 1, 2, 3, and 4 or chromatographically purified collagenase (Code: CLSPA); the latter usually combined with secondary enzymes such as elastase, hyaluronidase, etc. For best results, the precise mixture of proteolytic activities must be tailored to the tissue to be dissociated. Correlations between type and effectiveness with different tissues have been good, but not perfect, and may be dependent partly on parameters of use and objectives as well as lot-to-lot variations. For more information see the Collagenase Sampling Program information. Worthington also publishes a Tissue Dissociation Guidewhich provides additional information regarding the enzymes used for these applications and specific references for numerous cell and tissue types.
Unit Definition: One Unit releases one micromole of L-leucine equivalents from collagen in 5 hours at 37°C, pH 7.5
Brochure