Neutral protease (Dispase®) is a non-mammalian animal origin free (AOF) metallo, neutral protease, purified by methods developed at Worthington. Its mild proteolytic action makes the enzyme especially suitable for the preparation of primary cells and secondary (subcultivation) cell culture, since it is gentle on cell membranes. This protease is also used as a secondary enzyme in cell isolation and tissue dissociation applications, commonly used with collagense.
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