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Deoxyribonuclease I, Filtered

Cat.No. Size Price Quantity Subtotal Buy
LS002058 11MG 來電價
LS002060 25MG 來電價

LS002058. CODE: DCLS. Filtered through a 0.22 micron membrane and lyophilized in vials. Material is not tested for pyrogenicity.

Products Description

Source: Bovine Pancreas

I.U.B.: 3.1.21.1

Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position. The average chain length of a limit digest is a tetranucleotide.

Uses: Worthington offers DNase at different levels of purity for different applications. DNase is used in tissue culture work to digest DNA from damaged cells thereby reducing viscosity, and removing membrane bound DNA fragments. Worthington Codes: DP and DCLS are suitable for these applications.

Stability/Storage: When properly stored, all grades of Worthington deoxyribonuclease are stable for 2-3 years. Recommended storage temperature for all grades of Worthington DNases is 2-8°C.

Unit Definition: 1 Unit causes an increase in absorbance at 260nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0. Note: Kunitz units as reported by other suppliers can be 2 to 4 times higher than Kunitz units as measured at Worthington. As measured at Worthington, one Kunitz unit digests 1µg of lambda DNA in 10 minutes at 37°C in 50mM Tris, 1mM Mg2+, 1mM Ca2+, pH 7.8 in a 50µl reaction. Correlation of digestion units with Kunitz units is different for other DNA and buffer systems.

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