>>可穩定建構GeCKO lentivirus libraries
Stability of GeCKO libraries in Endura cells.Lanes 1 and 2, Separate libraries were prepared in Lucigen Endura cells and grown at 37°C. Library 1 was re-amplified by growth at 32°C (Lane 3). Undesired recombination may become more pronounced at 37°C. Recombination becomes more pronounced by growth at 37°C (data not shown). Courtesy Dr. G. Winter, MIT Broad Inst.
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