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Endura™ Competent Cells

Cat.No. Size Price Quantity Subtotal Buy
60240-1 Chemically 12 rxns (DUO) 來電價
60240-2 Chemically 24 rxns (DUO) 來電價
60241-1 Chemically 12 rxns (SOLO) 來電價
60241-2 Chemically 24 rxns (SOLO) 來電價
60242-1 Electro 12 rxns (DUO) 來電價
60242-2 Electro 24 rxns (DUO) 來電價

Endura 的 genotype 設計,可使高度重複性的序列,穩定的轉殖於 E. coli 中,用來生產高覆蓋性的 Genomic-scale CRISPR Knock Out (GeCKO) lenvirus library 再適合不過了。

Products Description

EnduraTM特色  

1) 專為不穩定基因Cloning設計的勝任細胞
2) 可直接取代昂貴的Stbl3/Stbl4 (Invitrogen)及 SURE (Agilent) type 勝任細胞
3) 高效率: >1 × 107 cfu/µg (Chemical competent) or 1 × 1010 cfu/µg (Electro-competent)
4) 推薦建構 lentiviral libraries of CRISPR clones
5) 適合single-guide RNA lentiviral library construction

 

Lucigen 專業團隊研發的 EnduraTM勝任細胞, 有2014年發表於Science的paper背書1,在Recombination resistant strains中效率最高, 同時並榮獲CRISPR/Cas9 KO library 發明者的推薦,寫入 Lentiviral CRISPR protocol2。Endura 的genotype設計,可使高度重複性的CRISPR/Lentiviral序列,穩定的轉殖於E. coli中,以用來生產高覆蓋性的Genomic-scale CRISPR Knock Out (GeCKO) lentivirus library。

 

>>可穩定建構GeCKO lentivirus libraries
 
Stability of GeCKO libraries in Endura cells.Lanes 1 and 2, Separate libraries were prepared in Lucigen Endura cells and grown at 37°C. Library 1 was re-amplified by growth at 32°C (Lane 3). Undesired recombination may become more pronounced at 37°C. Recombination becomes more pronounced by growth at 37°C (data not shown). Courtesy Dr. G. Winter, MIT Broad Inst.


>>榮獲CRISPR/Cas9 KO library 發明者及多位專家學者的推薦

Reference:
1: Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells. Shalem et al. (2014). Science, Vol. 343 no. 6166 pp. 84-87.
2: Lentiviral CRISPR Protocol http://www.addgene.org/static/cms/files/GeCKOv2.0_library_amplification_protocol.pdf


 

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