Products Description
Overview
- Amplify DNA templates with high sensitivity
- Amplify single-copy and low abundance templates
- Nuclease-free, high purity reagents
Description
DNA amplification kit containing all reagents for PCR reactions except template and primer. Each kit includes 10 mM of each dNTP, 50X, 10X, Reaction Buffer with MgCl2, 10X Reaction Buffer without MgCl2, 25mM MgCl2, Taq DNA Polymerrase, 500 U (5 U/µL), Water, Sterile, Nuclease-Free, 2 x 1.5 mL. Sufficient material for 200 x 25 µL reactions.
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Information
Amplification using Taq DNA Polymerase. 0.5% TAE agarose gel run at 5 V/cm and stained with ethidium bromide. Lane 1: 1 kb DNA Ladder, Lane 2: 1.5 kb PCR product generated using 0.2 mM dNTPs and 2.0 U Taq DNA Polymerase. Lane 3 and 4: 5 and 8 kb PCR products generated using 0.25 mM dNTPs, 2.5 U Taq DNA Polymerase and 3% formamide. Lane 5-7: 10 kb,15 kb and 20 kb PCR products generated using 0.36 mM dNTPs, 2.5 U Taq DNA Polymerase and 3% formamide.