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- 酵素 Enzymes & Inhibitors
- Nucleic Acid Related Enzymes
- Bst DNA Polymerase, Exonuclease Minus
Bst DNA Polymerase, Exonuclease Minus
Products Description
- High strand displacement activity.
- Used in isothermal amplification and Next Generation Sequencing.
Purity: >99% pure by SDS-PAGE. No detectable DNA contamination. 10 µl of enzyme at 8 U/µl of the sample was tested for E. coli genomic DNA contamination by PCR amplifying with the E. coli 16S ribosomal primers.
Activity Determination: One unit catalyzes the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 65°C in 20 mM Tris-HCl pH 8.8, 10 mM (NH4)2SO4, 10 mM KCl, 2 mM MgSO4, 0.1 % Triton X-100, 30 nM M13mp18 ssDNA, 70 nM M13 sequencing primer(-47) 24 mer 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and and 33P]dCTP), and 0.1 mg/ml BSA.