Products Description
- 可靠的雙股DNA/RNA黏合能力
- 室溫作用只要30分鐘
- 大包裝,一管可進行750次反應
- 高純度, >99%
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
| T4 DNA Ligase Version |
Sizes |
Provided Buffer |
| 低濃度 (2 U/µL) |
1,500 U |
10X T4 DNA Ligase Buffer |
| |
7,500 U |
|
| 高濃度 (10 U/µL) |
1,500 U |
2X Rapid Ligation Buffer |
| |
7,500 U |
10X T4 DNA Ligase Buffer |
10X T4 DNA Ligase Buffer is composed of 500 mM Tris-HCI, 100 mM MgCl2, 50 mM dithiothreitol, 10 mM ATP, pH 7.6 @ 25 °C.
2X Rapid Ligation Buffer is composed of 132 mM Tris-HCI, 20 mM MgCl2, 2 mM dithiothreitol, 2 mM ATP, 15% PEG, pH 7.6 @ 25 °C.
Unit Definition: One Weiss Unit is defined as the amount of enzyme required to convert 1 nmol of 32P-labeled inorganic pyrophosphate into Norit adsorbable material in 20 minutes at 37 °C, using specified reaction conditions. Note: 1 Weiss Unit is approximately 67 cohesive end units.
Resources
