Products Description
Overview
- Highest sensitivity of VisiGlo™ substrates - ideal for detecting low-abundance protein
- Quantitative with broad dynamic range
- High signal-to-noise ratio
- Signal lasts hours - enables repeated exposures and imaging delays
- Versatile imaging with x-ray film, CCD- and fluorescence-based imagers
Detailed Description
AMRESCO’s VisiGlo™ Select HRP Chemiluminescent Substrate Kit combines a broad linear dynamic range and attomolar sensitivy to enable quantitation of low-abundance targets labeled with horseradish peroxidase (HRP). The high sensitivity and signal-to-noise ratio allow for target detection with minimal consumption of valuable protein and antibody reagents, thereby lowering experimental costs. Low-intensity signals undetectable with competing chemiluminescent substrates may be revealed as well, because background levels remain low even for lengthy exposure times . The signal generated by substrate incubation is stable for hours and may be captured by conventional x-ray film, CCD and fluorescence-based imaging systems.
Kit includes:
VisiGlo™ Select HRP Chemiluminescent Substrate Kit Solution A, 50 ml
VisiGlo™ Select HRP Chemiluminescent Substrate Kit Solution B, 50 ml
Sufficient for 1,000 cm2 of membrane
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Information
Sensitive HRP detection with VisiGlo™ Select HRP Chemiluminescent Substrate. Identical slot-blots with 10-fold serial dilutions of horseradish peroxidase (10 ng - 10 fg) were incubated separately in three chemiluminescent substrates for comparison; VisiGlo™ Select HRP Chemiluminescent Substrate Kit (1B1583, AMRESCO). Amersham™ ECL™ Select (GE) and SuperSignal® West Femto (Pierce). VisiGlo™ Select sensitivity was comparable to that of competing substrates, with detection of as little as 10 fg HRP.
VisiGlo™ Select provides a long-lasting chemiluminescent signal. A blot containing HRP bands was incubated 5 minutes with VisiGlo™ Select HRP Chemiluminescent Substrate and imaged at 0, 1 and 3 hours post-substrate incubation using the same exposure settings. Significant chemiluminescent signal was retained even after 3 hours.
Directions For Use