Protein EZ-Vision® is non-hazardous, flourescent reagent that produces instant visualization of protein bands upon UV illumination of SDS-PAGE gels. Supplied in a 4X loading buffer, Protein EZ-Vision® comigrates with the protein-SDS complex during electrophoresis. Post-run staining and destaining is completely eliminated and results can be visualized immediately after the run by placing the gel on a standard UV transilluminator. Available in 2 x 1 ml tubes (KIT) or in a 0.2 ml sample size.
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Sensitivity of Protein EZ-Vision® (A) compared to Coomassie® Blue sensitivity (B). Both figures were generated using the same gel comparison. Two-fold serial dilutions of bovine serum albumin (BSA) were separated via a 12.5% Laemmli SDS-PAGE gel using Protein EZ-Vision®, 4X as the sample loading buffer. Lane 7 is approximately 100ng protein. Immediate fluorescent visualization of protein post-electrophoresis was achieved with a 2 sec UV exposure using a SYBR® Green filter with the Syngene GBox-HR Gel Doc System (A). The same gel was post-stained with Coomassie® Blue protein stain for one hour and destained one hour (B).
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