Ideal for environments needing to reduce use of ethidium bromide
Recommended for denaturing agarose gels with formaldehyde
Sensitive to 150 ng of RNA
Compatible with downstream applications including Northern blots
Detailed Description
RNA EZ-Vision® is a non-mutagenic fluorescent dye for staining RNA bands resolved on denaturing agarose gels containing formaldehyde. Supplied in a 1.5X loading buffer, RNA EZ-Vision® dye stains RNA during the denaturation immediately prior to gel loading. The dye comigrates with the RNA samples during electrophoresis and provides immediate band visualization upon illumination with UV light. No post run staining or destaining is needed. The loading buffer includes formamide and bromophenol blue tracking dye. RNA EZ-Vision® Loading Buffer, 1.5X is best used with RNA at a concentration of ≥ 1 mg/ml. If the stock RNA concentration is < 1mg/ml, RNA EZ-Vision® should be used as a 2X solution instead of a 1X solution. Sample size includes 0.3 ml of RNA EZ-Vision® Loading Buffer.
Click on the Features tab to learn more about RNA EZ-Vision® Dye as Loading Buffer, 1.5X
Information
A non-mutagenic alternative to ethidium bromide for RNA
Comparison between ethidium bromide and RNA EZ-Vision®. Total RNA was purified from K562 cells with Ribozol™ RNA Extraction Reagent.Samples were denatured at 65°C for 10 minutes in RNA EZ-Vision® loading buffer containing either 0.003% ethidium bromide or RNA EZ-Vision® dye. The denatured RNA was loaded onto a 2% formaldehyde denaturing agarose gel (Agarose I™, Code: 0710) and resolved for 1.5 hours at 5.1 V/cm. Bands were visualized by UV illumination and image capture was performed with a Syngene GBox-HR Gel Doc System with a SYBR® Green filter.
SDS