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EZ-Vision® is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon UV illumination of agarose gels. EZ-Vision® is non-mutagenic and non-toxic with no hazardous shipping, handling or disposal costs. Supplied in AMRESCO’s 6X DNA Loading Buffer, EZ-Vision® forms a tight complex with the sample DNA and co-migrates with it during electrophoresis. Post-run staining and destaining is completely eliminated and results can be visualized immediately after the run by placing the gel on a standard UV transilluminator. It is ideal for applications needing rapid DNA band visualization and for environments requiring a safe, non-hazardous alternative to Ethidium Bromide. 

EZ-Vision® is available in 3 versions that differ only by the tracking dyes included in the loading buffer. EZ-Vision® Three contains 3 tracking dyes that migrate at 4,000 bp, 400 bp, and 10 bp. EZ-Vision® Two contains 2 tracking dyes that migrate at 4,000 bp and 400 bp. EZ-Vision® One contains only a single fast-running tracking dye that migrates at approximately 10 bp in a 1% agarose gel.

 

EZ-Vision® One, Two and Three. Left image: 1% TAE agarose gel showing the fluorescence of AMRESCO’s 1kb Ladder (K180-250μl) stained with EZVision® One (lane 1), EZ-Vision® Two (lane 2) and EZ-Vision® Three (lane3) captured with Syngene GBox-HR Gel Doc Systemusing SP filter selection. Right image: Digital camera photograph of the same gel as left image, showing the colors and migration position of the one fast migrating tracking dye of EZ-Vision® One (lane 1), the two tracking dyes of EZ-Vision® Two (lane2), and the three tracking dyes of EZ-Vision® Three (lane 3).

EZ-Vision® sensitivity compared to Ethidium Bromide. DNA MW Marker, 1KB Ladder (K181) was mixed with EZ-Vision® Two, DNA Dye as Loading Buffer, 6X or a loading buffer containing 0.5 µg/mL ethidium bromide.  After electrophoresis, the gel was visualized on a standard UV illuminator.  

 

EZ-Vision® Environmental Toxicity Testing. EZ-Vision® environmental hazard testing was determined by the CCR Title 22 Fathead Minnow Hazardous Waste Screen Bioassay. Both EZ-Vision® Two and EZ-Vision® Three were determined non-hazardous with LC50 > 750 mg/l.  Additional information. 
 

Relative migration of prestained samples of dsDNA in 1% agarose gel. The data shows that EZ-Vision® has much less effect on DNA migration than Ethidium Bromide or Competitor 2 Green Dye. Samples stained with EZ-Vision® are not impeded and migrate similar to dsDNA that is stained post electrophoresis.