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LavaLAMP™ RNA Master Mix

Cat.No. Size Price Quantity Subtotal Buy
30086-1 200 Reactions 來電價
30087-1 with dye 200 Reactions 來電價
30078-2 Dye 200 Reactions 來電價

Products Description

LavaLAMP™RNA Master Mix

使用這種易於使用的RNA LAMP(RT-LAMP)Master Mix進行靈敏的RNA檢測

特色:
  • 等溫擴增:可利用簡化的儀器促進擴增反應
  • Master Mix格式:簡化反應進行,同時減少潛在的人為操作錯誤
  • 優化的條件:使用者只需關注兩個關鍵反應參數 - primer設計和反應溫度
  • 所有成分都是凍乾兼容的Lyophilization-ready

The LavaLAMP™ RNA Master Mix is built around a proprietary DNA polymerase with strand displacement activity that works on both RNA and DNA templates. These enzyme features make it possible to perform RNA LAMP (loop-mediate isothermal amplification starting with RNA targets/templates) in which the RNA target is first converted into cDNA and then that cDNA acts as template for additional LAMP, all using a single DNA polymerase. By combining the strong activity of this enzyme with an optimized master mix formulation, we’ve produced an easy-to-use system that enables fast, sensitive detection of specific RNA targets.

Simplified Reaction Set Up and Optimization with a Master Mix



Figure 1. The differences in RNA LAMP reaction set up and potential optimization parameters when using a master mix vs. a component kit format.  Panel A illustrates the components added to a RNA LAMP reaction (left) when using the LavaLAMP™ RNA Master Mix and the various parameters than can be optimized (right). Panel B illustrates reaction setup (left) and potential optimization parameters (right) when using a standard RNA LAMP component kit. Bold text indicates components or parameters common to both systems.

Quicker RNA Detection with LavaLAMP™ RNA Master Mix


 

Figure 2. Loop-mediated isothermal amplification (LAMP) with real-time fluorescent detection of amplified products. RNA LAMP reactions (6 replicates per condition) were set up using the indicated kits according to manufacturer’s recommendations. Target RNA (MS2 bacteriophage) at varying input amounts, MS2-specific RNA LAMP primers, and Green Fluorescent Dye (LavaLAMP™ RNA Master Mix Kit) were included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at the following temperatures: LavaLAMP; 68°C; other kits at the recommended 65°C and fluorescence was measured over 60 minutes to determine the TTR. NTC denotes No Target Control.


Faster Zika Virus RNA Detection with LavaLAMP™ RNA Master Mix



Figure 3: RNA LAMP-based detection of Zika Virus RNA. RNA LAMP reactions (6 replicates per condition) were set up using the indicated kits according to manufacturer’s recommendations. Target Zika virus RNA at varying input amounts, Zika-specific RNA LAMP primers, and Green Fluorescent Dye (LavaLAMP™ RNA Master Mix Kit) were included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at the following temperatures: LavaLAMP; 68°C; WarmStart® LAMP Kit; 65°C, and fluorescence was measured over 60 minutes to determine the TTR. NTC denotes No Target Control.

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Information

Both LavaLAMP™ RNA Master Mix kits contain: LavaLAMP™ RNA Master Mix, RNA Positive Control LAMP Primer Mix, and RNA Positive Control. The LavaLAMP™ RNA Master Mix with Dye also contains Green Fluorescent Dye for fluorescent detection of amplified DNA. The Green Fluorescent Dye is also available separately.

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