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Products Description

Probe 1-Step Go is a universal probe kit designed for fast, highly specific and ultra-sensitive probe-based real-time RT-PCR. We use the latest developments in reverse transcriptase technology and buffer chemistry to give efficient cDNA synthesis and real-time PCR in a single tube.
 

Figure 1. High efficiency and broad dynamic range
		TaqMan probe amplification traces of mouse gene ACTB using mouse liver total RNA as template in triplicate. Template concentrations are 10x serial dilutions ranging from 10pg to 1µg total RNA per 20µl reaction. Cycling conditions were 45°C 10min, 95°C 3min, then 45 cycles of 95°C 10s, 60°C 30s. qPCRBIO Probe 1-Step Go shows high efficiency over a broad dynamic range.

Figure 2. qPCRBIO Probe 1-Step Go in multiplex
		Four mouse housekeeping genes were amplified simultaneously in a single multiplex reaction. 1μg of mouse liver total RNA was used as template. Amplification was detected using TaqMan probes in the following gene/probe combinations: B2MG/HEX, ACTB/Cy5, GAPDH/FAM, and ACTG/TexasRed. Cycling conditions were 45°C 10min, 95°C 3min, then 45 cycles of 95°C 10s, 60°C 30s. This demonstrates that the qPCRBIO Probe 1-Step Go mix can be used to quantify and compare expression levels of multiple genes in a single reaction.