特色:
HRM Mix使用新型SyGreen 2 嵌入染料。不抑制PCR，是一種飽和的第3代染料，對於準確的SNP基因分型非常重要。與第一代染料不同，SyGreen 2可以高濃度使用，對AT或富含GC的序列無差異的親和力。結合Taq和先進緩衝化學的最新進展技術，提供市場領先的性能。qPCRBIO HRM Mix即使在第IV級SNP上也能提供準確的基因分型。
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Class I SNP Three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of Factor V gene. The primers flanked a class I single nucleotide polymorphism (1691G/A). After amplification the products were subjected to HRM analysis. The 3 traces show clear allele calling of a class I SNP. The black trace is for homozygous A, the purple trace homozygous T and the green trace heterozygous green.
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