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qScript XLT 1-Step RT-qPCR ToughMix

Cat.No. Size Price Quantity Subtotal Buy
95132-100 (no rox) 100 x 20 μL rxns (1 x 1 mL) 來電價
95132-500 (no rox) 500 x 20 μL rxns (5 x 1 mL) 來電價
95132-02K (no rox) 2000 x 20 μL rxns (1 x 20 mL) 來電價
95134-100 (low rox) 100 x 20 μL rxns (1 x 1 mL) 來電價
95134-500 (low rox) 500 x 20 μL rxns (5 x 1 mL) 來電價
95134-02K (low rox) 2000 x 20 μL rxns (1 x 20 mL) 來電價
95133-100 (rox) 100 x 20 μL rxns (1 x 1 mL) 來電價
95133-500 (rox) 500 x 20 μL rxns (5 x 1 mL) 來電價
95133-02K (rox) 2000 x 20 μL rxns (1 x 20 mL) 來電價

Features & Benefits

 
  • Tough Tested - Overcomes common inhibitors including polysacharides, heme/hemoglobin, humic acid, melanin
  • Flexible - Use fast or standard qPCR cycling conditions
  • Broad dynamic range- Reliable data from your precious samples every time
  • Multiplexing enabled - Supports highly sensitive detection for up to four targets

Products Description

Description


qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5'-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters. This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies. Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.


Instrument Capability


ROX
  • Applied Biosystems 5700
  • Applied Biosystems 7000
  • Applied Biosystems 7300
  • Applied Biosystems 7700
  • Applied Biosystems 7900
  • Applied Biosystems 7900HT
  • Applied Biosystems 7900 HT Fast
  • Applied Biosystems StepOne™
  • Applied Biosystems StepOnePlus™

Low ROX
  • Applied Biosystems 7500
  • Applied Biosystems 7500 Fast
  • Stratagene Mx3000P®
  • Stratagene Mx3005P™
  • Stratagene Mx4000™
  • Applied Biosystems ViiA 7
  • Applied Biosystems QuantStudio™
  • Agilent AriaMx
  • Douglas Scientific IntelliQube®
  • QIAGEN Rotor-Gene Q

No ROX
  • Quantabio Q
  • BioRad CFX
  • Roche LightCycler 480
  • Other

Bio-Rad iCycler iQ systems
  • BioRad iCycler iQ™
  • BioRad MyiQ™
  • BioRad iQ™5

Information

Contents

 

2X concentrated One-Step Master Mix containing:

  • Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
  • qScript XLT reverse transcriptase
  • RNase inhibitor protein
  • AccuStart II Taq DNA Polymerase
  • Inert AccuVue™ plate loading dye
  • Proprietary enzyme stabilizers and performance-enhancing additives
  • Titrated reference dye (if applicable)
 

Storage & Handling


Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt. Repeated freezing and thawing does not affect RT-qPCR performance. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.