qScript cDNA SuperMix is a sensitive and easy-to-use 1-tube reagent for first-strand cDNA synthesis that combines a highly-modified RNAse H+ mutant of M-MLV together with ribonuclease inhibitor protein (RIP) in a rigorously optimized formulation for real-time qPCR applications.
The stabilized SuperMix formulation has been rigorously optimized to deliver sensitive, linear assay performance across a spectrum of relative rare and abundance input RNA (10pg - 1ug).
qScript cDNA SuperMix reagent performance is unaffected by repetitive freeze/thaw cycling (>20 times), conferring greater ease-of-use and consistent assay performance.
Oligo (dT) and random primers are pre-blended in a precise ratio to provide equal representation of 5' and 3'-sequences for accurate gene expression quantification.
For gene-specific priming (GSP) or two-step RT-PCR of RNA exceeding 1kb total length, see our qScript Flex cDNA Kit.
Single-tube, 5X concentrated reagent containing:
Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP
Recombinant ribonuclease inhibitor protein (RIP)
qScript reverse transcriptase
Titrated concentrations of random hexamer and oligo(dT) primer
Proprietary enzyme stabilizers and performance-enhancing additives
Store components in a constant temperature freezer at -25°C to -15°C upon receipt. Repeated freezing and thawing does not affect functional performance. For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.
Thaw completely on ice then pulse vortex to mix and briefly spin-down to collect contents before opening tube. 5X concentrated reagent is viscous. Do not immerse pipet tip below liquid surface when drawing out material.
User Manual