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Products Description

產品特點:

• 75 ng to 1 µg of sheared/fragmented DNA input
• Increases number of usable samples without having to add PCR

PCR-free

• No chance of introducing any PCR amplification bias
• More uniform sequencing coverage of each sample

Optimized end-repair, A-tailing, ligation and reaction format

• Higher percentage of DNA fragments are sequenceable
• More complex libraries and better sequencing data
• Better multiplexing due to more data produced per sample

Fast and efficient protocol

• DNA libraries in ~2 hours = faster onto the sequencer

高效建庫能力

Figure 1. Percentage of library DNA with correctly ligated adaptors measured by qPCR. Duplicate libraries were prepped per kit/organism (Human, Staphylococcus aureus, Rhodobacter sphaeroides (1 library only), and E. coli) according to the manufacturer’s recommended input amounts and protocols. Adaptor ligation efficiency was measured by qPCR using the KAPA Library Quantification Kit (Complete ROX Low, cat #KK4873) and matching amplified library as an internal standard.

建庫快速

Figure 2. Faster Protocol with Significantly Less Hands-on Time