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NxSeq® UltraLow DNA Library Kit, 12 Reactions and Single Indexing Kits

Cat.No. Size Price Quantity Subtotal Buy
15012-1 12 rxn 來電價
15100-1 NxSeq® Single Indexing Kit, Set A, 48 rxn (12 x 4 rxn) 來電價
15200-1 NxSeq® Single Indexing Kit, Set B, 48 rxn (12 x 4 rxn) 來電價

Lowest input, highest efficiency, Illumina-compatible DNA fragment library prep kit

Products Description

NxSeq® UltraLow DNA Library Kit

只需要最微量的檢體 即可得到最好的結果


產品特色:

  • High Quality Data: 極高的adaptor連接效率,可得到 sequencing depth 一致性及絕佳的基因覆蓋率
  • Sensitive:  檢體量兼容範圍寬50 pg~ 75 ng of sheared/fragmented DNA
  • Minimal Bias: 有力,統一的PCR擴增, 以提高基因覆蓋率的一致性
  • Flexible: 應用範圍廣,可用於de novo whole genome sequencing, resequencing, exome-seq, ChIP-seq and FFPE DNA檢體
  • Fast: 建庫只要3 小時
  • High Value: Cost-effective library and indexing kits which produce excellent sequencing results
  • 適用於Illumina機器

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Single Index Library Construction原理:
Figure 1. Mechanics of NxSeq® UltraLow Library Construction Using Single Indexing Primers. This workflow figure illustrates how DNA fragment libraries are constructed using the NxSeq® UltraLow Library Prep Kit and NxSeq Single Indexing Kits. Note that some PCR fragments and products are not shown to simplify the illustration.

 

Highest Efficiency Adaptor Ligation


 
Figure 2: Percentages of DNA fragments with correctly ligated adaptors measured by qPCR. Two independent sets of libraries were prepped per kit/organism (Staphylococcus aureus, Rhodobacter sphaeroides, and E. coli) according to the manufacturer’s recommended protocols using 1 ng of sheared genomic DNA. Prior to PCR amplification, adaptor ligation efficiency for each library was measured by triplicate qPCR assays using the KAPA Library Quantification Kit (Complete ROx Low, cat #KK4873) with a Lucigen-designed PCR primer set that binds to and amplifies all adaptor-ligated DNA fragments independent of the kit used. Standard P5 and P7 primers cannot be used because the Lucigen Universal Adaptor and the NEB adaptor do not contain P5 and P7 sequences prior to amplification. Efficiency data was normalized to the NxSeq UltraLow Library Kit data set (1.0) for each set of libraries and then the data libraries was averaged and plotted.


 

Minimal GC- or AT-bias with the NxSeq® UltraLow DNA Library Kit


Figure 3. Sequencing bias analysis for three different organisms with varying GC content.Tripilcate genomic DNA fragment libraries were generated from gDNA of three organisms with varying GC content (Staphylococcus aureus, 24% GC; B - E. coli K12, 50% GC; and C - Rhodobacter sphaeroides, 68% GC) using the Lucigen NxSeq® UltraLow DNA Library Kit, Kapa Hyper Prep Kit or the NEB NEBNext Ultra II Kit according to the manufacturer’s recommended protocols using 1 ng aliquots of the same mechanically sheared genomic DNA samples. The final libraries were quantitated and diluted to 2 nM based on Bioanalyzer (size) and Qubit fluorometer (DNA quantity) analyses. The diluted libraries were then pooled and sequenced on a MiSeq using 2 × 150 bp chemistry. Normalized coverage was calculated as the (average coverage of all windows with x% GC content) divided by the (overall average coverage) and the data from each set of replicates was averaged and presented. The blue area represents the percent of each genome with the indicated GC content.
 

Fast, Streamlined Library Prep


Figure 4. Comparison of multiple library prep workflows and timing. The workflows illustrated correspond to the following kits: Lucigen, NxSeq® UltraLow DNA Library Kit; Illumina, TruSeq Nano DNA LT Library Preparation Kit; Kapa, Hyper Prep Kit with KAPA Library Amplification Primer Mix; NEB, NEBNext Ultra II DNA Library Prep Kit for Illumina.

 

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