特色:
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EconoTaq的QC規格非常嚴格：
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 Figure 1. High purity of EconoTaq DNA Polymerase (SDS PAGE). Lane 1, marker; Lane 2, Lucigen EconoTaq DNA Polymerase. |
EconoTaq性能
如圖2和圖3所示，Lucigen的EconoTaq DNA聚合酶在常規PCR中表現出與其他比較昂貴的Taq同等效能或更好。EconoTaq在DNA amplification與另一標準PCR Tfl DNA聚合酶一樣（圖4）。且EconoTaq的非特異性背景要低得多（圖4中比較EconoTaq和Tfl的“+”和“ - ” lanes）。EconoTaq DNA聚合酶還具有相當高的批次間重複性（圖2和4）
 Figure 2. Taq DNA polymerase from Promega and New England Biolabs were compared to Lucigen’s EconoTaq DNA Polymerase (2 different lots) in amplifying the ampicillin gene (0.8 kb) in a pUC19 vector. (–), no DNA. (+), DNA added (40 ng). MW, 1 kb ladder.
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Figure 3. EconoTaq vs. AmpliTaq® (Applied Biosystems) DNA polymerase in genotyping. All PCR reactions were performed in a RoboCycler 96 (Stratagene). Hip1 genotyping was performed using the following PCR conditions: 94°C for 1min, 35 cycles of 94°C for 30sec, 62°C for 60sec, 72°C for 90sec, and 72°C for 7min. Shh, Cdo and Gas1 genotyping were performed using the following PCR conditions: 94°C for 2min, 35 cycles of 94°C for 60sec, 65°C for 60sec, 72°C for 90sec, and 72°C for 7min. All PCR reactions contained final concentrations of 1 µM of each primer, 200 µM dNTPs, 1X cresol red loading dye, and 1U of the indicated Taq polymerase. Sequences for all PCR primers have been previously published (references available).
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 Figure 4. PCR amplification was performed under standard conditions using three different lots of EconoTaq DNA Polymerase and buffer, or duplicate reactions with Tfl DNA polymerase and buffer (Promega). Reactions contained primers specific for the 16S ribosomal RNA gene, with Bacillus genomic DNA (+) or no DNA (-) as a template (1450 bp product expected).
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Concentration: 5 units/µl. One unit catalyzes the incorporation of 10 nmol of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP dCTP (a mix of unlabeled and [33P] dCTP), 10 µg of activated calf thymus DNA, and 0.1 mg/ml BSA.
Storage Buffer: 10 mM Tris-HCl (pH 7.5), 100 mM KCl, 0.1% Triton X-100, 0.1 mM EDTA, 1mM DTT, and 50% glycerol.
10X Reaction Buffer:100mM Tris-HCl (pH 9.0), 500 mM KCl, 1% Triton X-100, and with or without 15mM MgCl2.
PCR Activity: EconoTaq DNA Polymerase is tested in DNA amplification using a variety of templates and primers.
Activity Determination: One unit of EconoTaq DNA Polymerase catalyzes the incorporation of 10 nmoles of dNTP into acid-insoluble material in 30 minutes at 70°C in 50 mM Tris-HCl (pH 9.0), 50 mM NaCl, 5 mM MgCl2, 200 µM dGTP, dATP, dTTP, dCTP (a mix of unlabeled and [33P]dCTP), 10 µg Activated Calf Thymus DNA, and 0.1 mg/ml BSA.
Manual Econo Taq