特色:
以抗體的 HoT Start Taq + 優化的 buffer
加熱至 95°C 才有活性，65°C 以下沒有活性,可以避免 primer dimer 和非專一性的擴增
適用於廣泛的 templates 包括 GC and AT rich
適合用來進行 Low-copy-number templates 的 PCR 實驗。
PCR 片段可達6kb
另有2X Master Premix & 2X Master Premix含Dye形式，簡便快速，降低 PCR 污染機率
應用: Genotyping • High throughput PCR • Low copy template detection • Standard and fast PCR • Routine and multiplex PCR • TA cloning • PCR direct from blood • Colony PCR • PCR of methylated DNA for bi-sulphite sequencing • “Difficult” PCR - GC/AT rich DNA
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Figure 1. 與競爭廠牌比較. Shows the amplification of a 1kb fragment of Beta-Actin under standard cycling conditions. Primer extension is prevented during reaction set up and first temperature ramp. Primer dimer amplification diverts DNA polymerase activity from the amplicon of interest and reduces sensitivity in the assay. The top row is PCRBIO HS Taq DNA Polymerase and the 2nd row is an equivalent product from Kapa Biosystems. |
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Figure 2. 可室溫存放. Shows no change in activity is detected in PCRBIO HS Taq DNA Polymerase after 28 days at 4˚C, 25˚C and 37˚C. |
Datasheet